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Endothelin converting enzyme 1
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Mca-RPPGFSAFK(Dnp)-OH
Mca-RPPGFSAFK(Dnp)-OH
Cat. No. PS-050
Structure:
Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)
Other Names:
Mca-(Ala7,Lys(Dnp)9)-bradykinin
Activity:
FRET substrate for endothelin-converting enzyme-1 (ECE-1)
Mca-RPPGFSAFK(Dnp)-OH, also known as Mca-(Ala7,Lys(Dnp)9)-bradykinin is a very sensitive fluorescent substrate for endothelin-converting enzyme-1 (ECE-1), the membrane-bound zinc metallopeptidase that is related to neprilysin. Mca-RPPGFSAFK(Dnp)-OH can also be used for assaying ACE1, ACE2, BACE, cathepsin A, cathepsin X/Z, ECE-1, ECE-2, MMP-2, MMP9, neprilysin, insulinase and thermolysin (TLN), although it is not a substrate for MMP1. Mca-RPPGFSAFK(Dnp)-OH substrate contains a highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group.
Johnson and Ahn (2000) Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. 286(1) 112 PMID: 11038281
Bruce et al (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. 275(22) 5607 PMID: 18959747
Nelson et al (2009) Reduction of β-Amyloid Levels by Novel Protein Kinase Cϵ Activators. J. Biol. Chem. 284 34514 PMID: 19850930
Related areas
All endothelin converting enzyme 1 modulators >
All fluorescent labelled peptides >
All substrate peptides >
Technical Data
| Structure | Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp) |
| CAS Number | 323577-36-2 |
| Molecular Weight | 1388.5 |
| Formula | C66H81N15O19 |
| Sequence | Mca-RPPGFSAFK(Dnp) |
| Modifications | 7-methoxycoumarin (Mca) at the N terminal, Lysine 9 has 2,4-dinitrophenyl (Dnp) attached |
Solubility and Storage
| Solubility | Soluble in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
Batch Specific Data
| Purity | >95% by HPLC |
| Safety Data Sheet | Mca-RPPGFSAFK(Dnp)-OH MSDS_602bfa7aee0a9.pdf |
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Recent citations
A new publication from the University of Ljubljana uses MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, the FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro), to determine the inhibitory potential of plant polyphenols
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