Custom antibodies

We offer a custom antibody service.  Please bear the following in mind when considering your antibody requirements.  We will be pleased to discuss your project in confidence with you at any time.

Peptide Antigen Selection

Peptide antigens must be carefully selected to offer the best chance of raising antibodies which will recognise the target protein whilst avoiding cross-reactivity with proteins containing potentially homologous protein sequences.  Isca Biochemicals produces high quality, fully characterised peptides for antibody production.

Synthesis and Conjugation

Synthetic peptides are generally too small to trigger an immune response, so peptide antigens must be conjugated to a carrier protein before they are used in immunisation.  Careful attention to conjugation chemistry is required to present the peptide appropriately.

Carrier proteins

KLH, OVA, BSA and TGB are regularly employed to conjugate synthetic peptides via a range of chemistries.  A cysteine can be routinely added to either the N-terminus or C-terminus of the peptide to allowan MBS-mediated optimised coupling procedure.  Typically produced in rabbits, goat or hen, polyclonal immune serum contains a pool of antibodies with a variety of chemicals and physical properties enabling recognition of multiple epitopes of the immunised antigen.  Polyclonal antibodies will exhibit batch to batch variability between animals.

Monoclonal Antibody Production

Monoclonal antibodies are typically produced from a single B-cell of immunised mice.  By fusion of the B-cell and a myeloma cell, a hybridoma cell line is produced which produces a single antibody conformation recognising a single protein epitope.  Monoclonal antibodies are highly homogeneous, typically exhibiting much lower cross reactivity than polyclonal antibodies and are much more amenable to large-scale synthesis for use in diagnostic kits.  Monoclonal antibodies are the preferred choice for quantitative applications.


Please contact us with your requirements - we look forward to hearing from you



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Contact Information

To place an order, or for customer services, order processing or technical support please contact us on:

UK, Europe and RoW
Tel: (+44) 01392 422205Fax: (+44) 01392 279510
[email protected]

USA and Canada

Tel: 610-994-1134

Toll Free: 855-FOCUS21 (855-362-8721)

Fax: 610-465-9100

[email protected] 


France

CliniSciences
Tel : +33 9 77 40 09 09
Fax :  +33 9 77 40 10 11
Orders and customer support : [email protected]


Germany, Switzerland, Austria

BIOZOL Diagnostics 

Tel: +49 (089) 3799 666-6

Fax: +49 (089) 3799 666-99
Orders, Technical and Customer support: [email protected]


Italy
Labospace
Tel: +39 02 35980841
Fax +39 02 359808004
Orders: [email protected]


Spain and Portugal
Abyntek Biopharma
Tel: +34 94 404 80 80

Fax: +34 94 404 80 81
Orders: [email protected]


China

BIOHUB INTERNATIONAL 

上海起发实验试剂有限公司

Tel: +86-15921799099

Tel: +86-021-50724187

Fax: +86-021-50724961

Orders: [email protected]

India
Biotechno Labs
Tel: +91-8860924629
07291852429
Orders, Technical and Customer support: [email protected]


Japan

Namiki Shoji Co.
Tel:+81-050-5527-9850
Fax:+81-3-3352-2196
email: [email protected]


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