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Matrix metalloproteases
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Mca-PLGL-Dap(Dnp)-AR-NH2
Mca-PLGL-Dap(Dnp)-AR-NH2
Cat. No. PS-010
Structure:
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
Activity:
FRET substrate for matrix metalloproteinases
Mca-PLGL-Dap(Dnp)-AR-NH2 is a sensitive synthetic peptide FRET substrate for the determination of matrix metalloproteinase (MMP) activity. Protease cleavage at the -Gly-Leu- bond releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a 190 fold increase in fluorescence intensity. Mca-PLGL-Dap(Dnp)-AR-NH2 is suitable for use in continuous assays at enzyme concentrations comparable to those used with macromolecular substrates and also for the assessment of total matrix metalloproteinase in crude preparations.
Knight et al (1992) A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett. 296(3) 263 PMID: 1537400
López-Pelegrín et al (2013) A novel family of soluble minimal scaffolds provides structural insight into the catalytic domains of integral membrane metallopeptidases. J. Biol. Chem. 288(29) 21279 PMID: 23733187
Related areas
All matrix metalloproteases modulators >
All fluorescent labelled peptides >
All substrate peptides >
Technical Data
| Structure | Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
| CAS Number | 140430-53-1 |
| Molecular Weight | 1093.2 |
| Formula | C49H68N14O15 |
| Sequence | Mca-PLGLX(Dnp)AR-NH2 |
| Modifications | Mca (7-methoxycoumarin) on N terminal, C terminal is amidated, X is diaminopropionic acid substituted with 2,4-dinitrophenyl (Dnp) |
Solubility and Storage
| Solubility | Soluble in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
Batch Specific Data
| Purity | >95% by HPLC |
| Safety Data Sheet | Mca-PLGL-Dap(Dnp)-AR-NH2 MSDS_602bf968e8a28.pdf |
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Recent citations
A new publication from the University of Ljubljana uses MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, the FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro), to determine the inhibitory potential of plant polyphenols
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