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MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2

Cat. No. CV-070

Structure:

Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2

Other Names:

SARS Main Protease FRET Substrate

Activity:

FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro)
Price Qty
1 mg    $ 259.00
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4–P5' of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. Enzmatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a large increase in fluorescence intensity.

Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 PMID: 16128623

Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 PMID: 18562531

Bahun et al (2021) Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols, Food Chemistry 131594 https://doi.org/10.1016/j.foodchem.2021.131594.


Technical Data

Structure Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2
CAS Number 932391-88-3
Molecular Weight 1514.7
Formula C69H99N19O15
Sequence Mca-AVLQSGFRK(Dnp)K-NH2
Modifications N terminal 7-methoxycoumarin (Mca), C terminal amide, lysine 9 has 2,4-dinitrophenyl (Dnp) attached

Solubility and Storage

Solubility Soluble as a stock solution in DMSO
Appearance Freeze dried solid
Storage Store desiccated, frozen and in the dark

Batch Specific Data

Purity >95% by HPLC
Certificate of Analysis MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 0634A-210121_602d1393072f2.pdf
Safety Data Sheet MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 MSDS_602bf83a15c9c.pdf

Contact Information

To place an order, or for customer services, order processing or technical support please contact us on:

UK, Europe and RoW
Tel: (+44) 01392 422205Fax: (+44) 01392 279510
[email protected]

USA and Canada

Tel: 610-994-1134

Toll Free: 855-FOCUS21 (855-362-8721)

Fax: 610-465-9100

[email protected] 


France

CliniSciences
Tel : +33 9 77 40 09 09
Fax :  +33 9 77 40 10 11
Orders and customer support : [email protected]


Germany, Switzerland, Austria

BIOZOL Diagnostics 

Tel: +49 (089) 3799 666-6

Fax: +49 (089) 3799 666-99
Orders, Technical and Customer support: [email protected]


Italy
Labospace
Tel: +39 02 35980841
Fax +39 02 359808004
Orders: [email protected]


Spain and Portugal
Abyntek Biopharma
Tel: +34 94 404 80 80

Fax: +34 94 404 80 81
Orders: [email protected]


China

BIOHUB INTERNATIONAL 

上海起发实验试剂有限公司

Tel: +86-15921799099

Tel: +86-021-50724187

Fax: +86-021-50724961

Orders: [email protected]

India
Biotechno Labs
Tel: +91-8860924629
07291852429
Orders, Technical and Customer support: [email protected]


Japan

Namiki Shoji Co.
Tel:+81-050-5527-9850
Fax:+81-3-3352-2196
email: [email protected]


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