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Products
Pharmacology
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- Resources
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2
Cat. No. CV-070
Structure:
Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2
Other Names:
SARS Main Protease FRET Substrate
Activity:
FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro)
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4–P5' of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. Enzmatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a large increase in fluorescence intensity.
Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 PMID: 16128623
Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 PMID: 18562531
Bahun et al (2021) Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols, Food Chemistry 131594 https://doi.org/10.1016/j.foodchem.2021.131594.
Related areas
All serine protease modulators >
All fluorescent labelled peptides >
All substrate peptides >
All antivirals >
Technical Data
| Structure | Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2 |
| CAS Number | 932391-88-3 |
| Molecular Weight | 1514.7 |
| Formula | C69H99N19O20 |
| Sequence | Mca-AVLQSGFRK(Dnp)K-NH2 |
| Modifications | N terminal 7-methoxycoumarin (Mca), C terminal amide, lysine 9 has 2,4-dinitrophenyl (Dnp) attached |
Solubility and Storage
| Solubility | Soluble as a stock solution in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
Batch Specific Data
| Purity | >95% by HPLC |
| Certificate of Analysis | MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 0634A-210121_602d1393072f2.pdf |
| Safety Data Sheet | MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 MSDS_602bf83a15c9c.pdf |
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Recent citations
A new publication from the University of Ljubljana uses MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, the FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro), to determine the inhibitory potential of plant polyphenols
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