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Mca-KPLGL-Dap(Dnp)-AR-NH2

Cat. No. PS-060

Structure:

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

Activity:

FRET substrate for matrix metalloproteinases
Price Qty
1 mg    $ 165.00
Mca-KPLGL-Dap(Dnp)-AR-NH2 is a broad spectrum fluorogenic FRET substrate suitable for matrix metalloproteinases including intestinal collagenase (MMP-1), neutrophil collagenase (MMP-8) collagenase 3 (MMP-13), gelatinase A (MMP-2), gelatinase B (MMP-9), matrilysin (MMP-7) and MMP-17. Mca-KPLGL-Dap(Dnp)-AR-NH2 is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Mca-KPLGL-Dap(Dnp)-AR-NH2 was obtained by the addition of an N-terminal lysine to Mca-PLGL-Dap(Dnp)-AR-NH₂ (PS-040) which improves substrate properties. It contains the highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. Mca-KPLGL-Dap(Dnp)-AR-NH2 is fully water soluble and allows measurement of metalloproteinase activity in tissue culture conditions such as on the surface of cells.

Neumann et al (2004) Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme. Anal. Biochem. 328(2) 166 PMID: 15113693

Devy et al (2009) Selective inhibition of matrix metalloproteinase-14 blocks tumor growth, invasion, and angiogenesis. Cancer Res. 69(4) 1517 PMID: 19208838

Walsh and Damjanovski (2011) IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-β1 resulting in epithelial to mesenchymal transition. Cell. Commun. Signal 9 10 PMID: 21535875


Related areas

All matrix metalloproteases modulators >
All fluorescent labelled peptides >
All substrate peptides >


Technical Data

Structure Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
CAS Number 720710-69-0
Molecular Weight 1221.3
Formula C55H80N16O16
Sequence Mca-KPLGLX(Dnp)AR-NH2
Modifications 7-methoxycoumarin (Mca) at the N terminal, X is diaminopropionic acid substituted with 2,4-dinitrophenyl (Dnp), C terminal amide

Solubility and Storage

Solubility Soluble as a stock solution in DMSO
Appearance Freeze dried solid
Storage Store desiccated, frozen and in the dark

Batch Specific Data

Purity >95% by HPLC
Safety Data Sheet Mca-KPLGL-Dap(Dnp)-AR-NH2 MSDS_602bf8f48973d.pdf

Contact Information

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