Search
0
Items
Select
Search
MENU
Select

5-FAM-LPETGG

Cat. No. FP-020

Structure:

5-FAM-Leu-Pro-Glu-Thr-Gly-Gly-OH

Activity:

Sortase reagent
Price Qty
0.1 mg    $ 165.00

5-FAM-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label 5-FAM. 5-FAM-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. 5-FAM-LPETGG contains the a 5- carboxyfluorescein label, which is one of the most widely used green fluorescent reagents for in situ labeling of peptides, proteins and nucleotides.

Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem. 9(5) 802 PMID: 18297670

Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. 50 5024 PMID: 21538739

Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. 8(9) 1800 PMID: 23989674


Related areas

All protein-sorting transpeptidase modulators >
All fluorescent labelled peptides>


Technical Data

Structure 5-FAM-Leu-Pro-Glu-Thr-Gly-Gly-OH
Molecular Weight 930.83
Formula C45H50N6O16
Sequence 5-FAM-LPETGG
Modifications 5-FAM at N terminal

Solubility and Storage

Solubility Soluble in water
Appearance Freeze dried solid
Storage Store desiccated, frozen and in the dark

Batch Specific Data

Purity >95% by HPLC

Contact Information

To place an order, or for customer services, order processing or technical support please contact us on:

UK, Europe and RoW
Tel: (+44) 01392 422205
Fax: (+44) 01392 279510
info@iscabiochemicals.com

USA and Canada

Tel: 610-994-1134

Toll Free: 855-FOCUS21 (855-362-8721)

Fax: 610-465-9100

sales@focusbiomolecules.com 


France

CliniSciences
Tel : +33 9 77 40 09 09
Fax :  +33 9 77 40 10 11
Orders and customer support : info@clinisciences.com


Germany, Switzerland, Austria

BIOZOL Diagnostics 

Tel: +49 (089) 3799 666-6

Fax: +49 (089) 3799 666-99
Orders, Technical and Customer support: info@biozol.de


Italy
Labospace
Tel: +39 02 35980841
Fax +39 02 359808004
Orders: info@labospace.com


Spain and Portugal
Abyntek Biopharma
Tel: +34 94 404 80 80

Fax: +34 94 404 80 81
Orders: info@abyntek.com


China

BIOHUB INTERNATIONAL 

上海起发实验试剂有限公司

Tel: +86-15921799099

Tel: +86-021-50724187

Fax: +86-021-50724961

Orders: sale3@78bio.com

India
BTL Biotechno Labs Pvt Ltd
Tel: +91-8860924629
07291852429
Orders, Technical and Customer support: info@biotechnolabs.com


Japan

Namiki Shoji Co.
Tel:+81-050-5527-9850
Fax:+81-3-3352-2196
email:  globalbusiness1@namiki-s.co.jp


All distributors >