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5-FAM-LPETGG
Cat. No. FP-020
Structure:
Activity:
5-FAM-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label 5-FAM. 5-FAM-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. 5-FAM-LPETGG contains the a 5- carboxyfluorescein label, which is one of the most widely used green fluorescent reagents for in situ labeling of peptides, proteins and nucleotides.
Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem. 9(5) 802 PMID: 18297670
Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. 50 5024 PMID: 21538739
Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. 8(9) 1800 PMID: 23989674
Related areas
All protein-sorting transpeptidase modulators >
All fluorescent labelled peptides>
Technical Data
Structure | 5-FAM-Leu-Pro-Glu-Thr-Gly-Gly-OH |
Molecular Weight | 930.83 |
Formula | C45H50N6O16 |
Sequence | 5-FAM-LPETGG |
Modifications | 5-FAM at N terminal |
Solubility and Storage
Solubility | Soluble in water |
Appearance | Freeze dried solid |
Storage | Store desiccated, frozen and in the dark |
Batch Specific Data
Purity | >95% by HPLC |
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