TPCK

PI-220-500MG 500mg 75.00 GBP

Product specification

Sequence: C14H21ClN2O3S · HCl
MW: 351.85
Purity: ≥98% by TLC
Appearance: White solid
Formula: C17H18ClNO3S
CAS: 402-71-1
Solubility DMSO
Long Term Storage: We recommend storage desiccated, frozen and in the dark
Miscellaneous/General:

TPCK is an irreversible chymotrypsin-like serine protease inhibitor, acting as an alkylating agent that interferes with translation.  TPCK is useful for inhibiting chymotrypsin activity in trypsin preparations. TPCK also inhibits apoptosis in thymocytes and blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides.

Product Literature References

Fearnhead et al (1995) A pre-existing protease is a common effector of thymocyte apoptosis mediated by diverse stimuli, FEBS Lett. 357 242 doi: 10.1016/0014-5793(94)01367-A

Griscavage et al (1996) Inhibitors of the Proteasome Pathway Interfere with Induction of Nitric Oxide Synthase in Macrophages by Blocking Activation of Transcription Factor NF-kappa. Proc. Natl. Acad. Sci. USA 93(8) 1996, pp. 3308 www.jstor.org/stable/38955