PS-030-1MG 1mg 125.00 GBP
PS-030-5MG 5mg 425.00 GBP

Product specification

Sequence: Mca-YVADAP-K(Dnp)-OH
MW: 1145.1
Purity: >95% by HPLC
Appearance: Freeze dried solid
Formula: C53H64N10O19
CAS: 189696-01-3
Solubility Soluble in DMSO
Long Term Storage: We recommend storage desiccated, frozen and in the dark

Mca-YVADAP-K(Dnp)-OH, or Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH, is a highly fluorescent FRET substrate for the determination of caspase-1 (ICE) and caspase-1-like enzyme activities. Cleavage of Mca-YVADAPK(Dnp)-OH at the P1 Asp residue results in a continuous fluorescent assay which can be monitored at an emission wavelength of 392 nm. Caspase-3 (apopain, CPP-32) is only weakly active on this substrate.  Mca-YVADAP-K(Dnp)-OH is a specific substrate for renal ACE 2.

Product Literature References

Kondo et al (1997) Activation of distinct caspase-like proteases by Fas and reaper in Drosophila cells. Proc. Natl. Acad. Sci. 94(22) 11951 DOI: 10.1073/pnas.94.22.11951

Bekhouche et al (2010) Role of the netrin-like domain of procollagen C-proteinase enhancer-1 in the control of metalloproteinase activity. J. Biol. Chem. 285(21) 15950 doi:10.1074/jbc.M109.086447

Bijakowski et al (2012) Sizzled Is Unique among Secreted Frizzled-related Proteins for Its Ability to Specifically Inhibit Bone Morphogenetic Protein-1 (BMP-1)/Tolloid-like Proteinases. J. Biol. Chem. 287 33581 doi: 10.1074/jbc.M112.380816

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