PS-020-1MG 1mg 125.00 GBP
PS-020-5MG 5mg 425.00 GBP

Product specification

Sequence: Mca-SEVNLDAEFR-K(Dnp)-RR-NH2
MW: 2001.1
Purity: >95% by HPLC
Appearance: Freeze dried solid
Formula: C86H125N27O29
CAS: 438625-61-7
Solubility Soluble in DMSO
Long Term Storage: We recommend storage desiccated, frozen and in the dark

Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, also knowm as Mca-(Asn⁶⁷⁰,Leu⁶⁷¹)-Amyloid β/A4 Protein Precursor₇₇₀ (667-676)-Lys(Dnp)-Arg-Arg amide, is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site.  Cleavage  at -Leu-Asp- of Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

Product Literature References

Koike et al (1999) Thimet Oligopeptidase Cleaves the Full-Length Alzheimer Amyloid Precursor Protein at a β-Secretase Cleavage Site in COS Cells.  J. Biochem. 126(1) 235

Klaver et al (2010), Glycosaminoglycan‐induced activation of the β‐secretase (BACE1) of Alzheimer’s disease. J. Neurochem. 112 1552 doi:10.1111/j.1471-4159.2010.06571.x

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